Semi Prep HPLC Column, Bidentate C8, 4um, 100A. 10mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑SP250 Cogent Bidentate C8™ Semi‑Preparative HPLC Column is the longest and highest‑resolution semi‑prep format within the Bidentate C8™ lineup, designed for purification workflows that require maximum separation, extended retention, and the highest achievable fraction purity. Built on Cogent TYPE‑C™ silica hydride technology, this column provides excellent selectivity, fast equilibration, and very robust column lifetime under demanding preparative conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, delivering balanced retention ideal for hydrophobic molecules, metabolites, and amine‑containing analytes when C18 is overly retentive.
With a 10 mm internal diameter and extended 250 mm length, this column provides the highest plate count in the semi‑preparative C8™ family, enabling the separation of closely related compounds, structural analogs, or complex mixtures prior to fraction collection. The longer format increases interaction time, improving resolution and allowing tighter fraction cut windows.
System Note: A specialized semi‑preparative HPLC system (sufficient pump flow capacity, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size and length.
Why Choose the 10 × 250 mm Semi‑Prep Bidentate C8™ Format
This configuration is typically selected when:
Maximum purification resolution is required
Shorter semi‑prep columns (50–150 mm) do not fully resolve critical compounds
Large‑volume injections contain closely related impurities or isomers
C18 columns are too retentive or slow for target analytes
Highest possible fraction purity is required
The long column length dramatically increases interaction time and selectivity, while TYPE‑C™ silica hydride chemistry maintains fast equilibration and long service life even under aggressive purification conditions.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional preparative C8 columns
Very robust lifetime for repeated purification workflows
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Ideal alternative when C18 is overly retentive
Semi‑Preparative Geometry for Maximum Resolution
10 mm ID × 250 mm length
Highest plate count among semi‑preparative C8 columns
Enables tight fraction collection and improved purity
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑dominant mobile phases
Suitable for demanding purification methods
Typical Applications
Semi‑preparative purification requiring maximum resolution
Isolation of closely related or structurally similar compounds
Hydrophobic molecule purification
Metabolite and amine‑containing analyte isolation
Scale‑up from analytical or shorter semi‑prep methods
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable